Table 1.

Purification of Ado-PNP from M. smegmatis

Step	Total protein (mg)	Enzyme activity (nmol/mg·min)	Purification (fold)	Total activity (nmol/min)	Recovery (%)
Cell extract	98	0.06	1	5.9	100
HiTrap Q	3.3	0.4	7	1.4	20
HiTrap Phenyl	0.034	1.9	32	0.056	1
Superdex 200 PG	NDa	—b	>32	0.021	0.4

^aND, not detected.

^b—, not determined.