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Substrate specificity of M. smegmatis Ado-PNP
| Nucleoside (100 μM) | Enzyme activitya (pmol min−1 ml−1) |
|---|---|
| Purines | |
| Adenosine | 19 ± 2 |
| 2′-Deoxyadenosine | 11.28 ± 0.05 |
| Inosine | 8 ± 4 |
| Guanosine | 6 ± 1 |
| Xanthosine | − |
| 5′-Methylthioadenosine | − |
| S-Adenosyl-l-homocysteine | − |
| Adenosine analogs | |
| 2-Fluoroadenosine | 26.3 ± 0.4 |
| 2-Methyladenosine | 5.8 ± 0.5 |
| Pyrimidines | |
| Cytidine | − |
| Thymidine | − |
| Uridine | − |
Mean ± standard deviation of at least 3 determinations. −, no activity detected.
