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. 2011 Oct;193(20):5668–5674. doi: 10.1128/JB.05394-11

Table 3.

Apparent steady-state kinetic constants of M. smegmatis Ado-PNP

Substrate Cosubstrate Kma (μM) Vmaxa (pmol min−1 ml−1)
Nucleoside phosphorolysis
    Adenosine 50 mM phosphate 98 ± 6 450 ± 10
    Phosphate 200 μM adenosine 700 ± 100 350 ± 20
Nucleoside synthesis
    Adenine 200 μM ribose-1-phosphate 43 ± 7 1,360 ± 80
    Ribose-1-phosphate 200 μM adenine 70 ± 10 1,470 ± 90
a

Mean ± standard deviation of at least 3 determinations.