Fig. 1.

MPA preinfection alters IFN response to RV infection in primary CF and normal bronchial epithelial cells. Mucociliary-differentiated CF bronchial epithelial cells were infected apically with MPA or treated with PBS and incubated for 24 h. Cells were then infected with RV39 and incubated for another 24 h. Sham- or UV-RV-infected cells were used as controls. Total RNA was isolated from CF (A to C) or normal (D to F) cells, and mRNA levels of IFNs were measured by qPCR. IFN-λ1 protein levels in the basolateral medium from CF (G) and normal (H) cells were determined by ELISA. Cells infected with RV were harvested at 6, 12, or 24 h postinfection, and the viral titer was determined (I). Cells infected with RV or coinfected with MPA/RV and incubated for 24 h. Cells along with medium were harvested, and the viral titer was determined (J). Data represent the ranges and medians (horizontal lines) calculated from experiments performed with cells obtained from three CF and three healthy individuals in duplicate. *, different from sham-treated group (P ≤ 0.05); †, different from cells infected with RV alone (P ≤ 0.05) (ANOVA on ranks [A to H] or Mann-Whitney test [I]).