Fig. 10.

DPI treatment reverses the suppressive effect of MPA on RV-induced IFN responses in primary CF airway epithelial cells. Mucociliary differentiated primary CF airway epithelial cells were pretreated with 10 μM DPI for 12 h. Cells were then infected with MPA or treated with PBS in the presence of DPI, incubated for 24 h, and then infected with RV and further incubated for 24 h. Total RNA was used for determination of IFN mRNA expression (A to C). Cells along with medium were harvested, and the viral titer was determined (D). Data represent averages ± SD or ranges with medians calculated from two or three independent experiments performed in duplicate. *, different from UVRV-infected group (P ≤ 0.05); †, significantly different from cells infected with RV alone (P ≤ 0.05); ‡, different from cells not treated with DPI (P ≤ 0.05) (ANOVA). In some experiments, cells were harvested 6 h (for Akt) or 18 h (for IRF3) after RV infection, and phosphorylation of Akt and IRF3 was determined (E). Representative blot showing phosphorylation of Akt and IRF3 in the presence or absence of DPI.