Fig. 2.

MPA suppresses the IFN response to RV infection in IB3 cells but not in BEAS-2B cells. Confluent monolayer of immortalized CF airway epithelial (IB3) or normal (BEAS-2B) cells were infected with MPA or treated with medium and incubated for 16 h. Cells were then infected with RV, UV-RV, or an equal volume of sham and incubated for a further 24 h. Total RNA isolated from cells was used for determination of mRNA levels of IFNs and IL-8 (A, B, E, and F). Conditioned medium was used to determine IFN-λ₁ protein by ELISA (C and D). Cells along with the medium were harvested, and the viral titer was determined (G and H). Data represent averages ± SD calculated from four experiments performed in duplicate or the ranges and medians. *, different from sham-treated group (P ≤ 0.05); †, significantly different from cells infected with RV alone (P ≤ 0.05) (ANOVA [A to F] or Mann-Whitney test [G and H]).