Fig. 5.

MPA suppresses the RV-induced IFN response by inhibiting IRF3 and Akt phosphorylation in IB3 cells. IB3 or BEAS-2B cells were infected with MPA and RV as described above. Cells were harvested 4 h (for Akt) or 12 h (for IRF3) after RV infection and subjected to Western blot analysis with antibodies to p-IRF3 or total IRF3 (A) or p-Akt or total Akt (C). A representative blot from three or four independent experiments is presented in panels A and C. (B and D) Blots from replicate experiments were quantified by densitometry, and the density of p-IRF3 and p-Akt was normalized to that of total IRF3 and Akt, respectively, and expressed as a fold increase over results for the respective UV-RV-infected cells. *, different from sham-treated group (P ≤ 0.05); †, significantly different from cells infected with RV alone (P ≤ 0.05) (ANOVA).