Fig. 8.

Analysis of the target cells of the tricomponent complex. (a) Affinity of the COMP-Z (filled bars) for various human or mouse immunoglobulin (Ig) isotypes. The COMP coiled-coil domain devoid of the Z domain ligand (open bars) was used as a negative control. (b) Flow cytometry of the COMP-Z. Freshly isolated splenocytes were first double stained with an FITC-conjugated anti-CD19 or anti-CD3 antibody and with PE-conjugated COMP or COMP-Z and were then analyzed on a FACSCalibur flow cytometer.