Table 1.
Strains used in this study
| Strain | Relevant genotype, phenotype, or descriptiona | Source or reference |
|---|---|---|
| EDL933 | Wild-type O157:H7 | Alison O'Brien |
| EDL933 Strr | Spontaneous Strr | 51 |
| EDL933 Strr Nalr | Spontaneous Nalr | 51 |
| MG1655 | Wild type (CGSC 7740) | CGSCb |
| MG1655 Strr | Spontaneous Strr | 52 |
| MG1655 Strr Nalr | Spontaneous Nalr | 52 |
| VJS4322 | Δ(narP)253::Tn10d(cat) | Valley Stewart |
| VJS2458 | Δ(narXL)240::Knr | Valley Stewart |
| Derivatives of EDL933 Strr | ||
| cydAB mutant | Δ(cydAB)::cat | This study |
| cydDC mutant | Δ(cydDC)::cat | This study |
| dmsAB mutant | Δ(dmsAB)::cat | This study |
| frdA mutant | ΔfrdA::Knr | This study |
| napDA mutant | Δ(napDA)::cat | This study |
| narG mutant | ΔnarG::Knr | This study |
| narZ mutant | ΔnarZ::cat | This study |
| narG narZ mutant | ΔnarG ΔnarZ::cat | This study |
| narG narZ napDA mutant | ΔnarG ΔnarZ Δ(napDA)::cat | This study |
| ubiCA mutant | Δ(ubiCA)::cat | This study |
| ubiE mutant | ΔubiE::cat | This study |
| Derivatives of MG1655 Strr | ||
| cydAB mutant | Δ(cydAB)::cat | This study |
| cydDC mutant | Δ(cydDC)::cat | This study |
| dmsAB mutant | Δ(dmsAB)::cat | This study |
| dmsAB ynfEFGH mutant | Δ(dmsAB) Δ(ynfEH)::cat | This study |
| frdA mutant | ΔfrdA::Knr | This study |
| napDA mutant | Δ(napDA)::cat | This study |
| narG mutant | ΔnarG::Knr | This study |
| narZ mutant | ΔnarZ::cat | This study |
| narG narZ mutant | ΔnarG ΔnarZ::cat | This study |
| narG narZ napDA mutant | ΔnarG ΔnarZ Δ(napDA)::cat | This study |
| narP mutant | Δ(narP)253::Tn10d(cat) | This study |
| narXL mutant | Δ(narXL)240::Knr | This study |
| nirBD mutant | Δ(nirB-nirD)::cat | This study |
| nrfA mutant | ΔnirfA::cat | This study |
| nrfA nirBD mutant | ΔnirfA Δ(nirBD)::cat | This study |
| torCA mutant | Δ(torCA)::cat | This study |
| torYZ mutant | Δ(torYZ)::cat | This study |
| torCA torYZ mutant | Δ(torCA) Δ(torYZ)::cat | This study |
| torCA torYZ dmsAB mutant | Δ(torCA) Δ(torYZ) Δ(dmsAB)::cat | This study |
| ubiCA mutant | Δ(ubiCA)::cat | This study |
| ubiE mutant | ΔubiE::cat | This study |
a
Nalr, nalidixic acid resistance; Strr, streptomycin resistance; Knr, kanamycin resistance.
b
E. coli Genetic Stock Culture Collection, Yale University.