Fig. 3.

Amino acid utilization by Filifactor alocis strains. Amino acids arginine, lysine, valine, threonine, isoleucine, glycine, and cystine were used at 100 μM concentration in BHI medium supplemented with hemin (5 μg/ml), vitamin K (0.5 μg/ml), and cysteine (0.1%). The cultures were grown for 8 h, and OD₆₀₀ was determined. F. alocis grown without any amino acid supplementation was used as a control. The data are means ± SD of three independent experiments. Arginine supplementation was compared statistically with the other amino acids used.