Table 1.
Oligonucleotide primers used for mutant production
| Gene | Oligonucleotide sequences of primersa | Enzyme | Resistance cassette |
|---|---|---|---|
| argA | TCACTCGAGGCAAAGAGGTGTGCCGTG | XhoI | Km |
| GCCGCTGGGCCGCTGGGGTACCARGACGGCGTGG ATT | KpnI | ||
| CTCGCCTCGTGCCATGGTACCCCAGCGGC | KpnI | ||
| CGCAGATCTTAACCCTAAATCCGCCATCA | BglII | ||
| potG | TCACTCGAGACGAAAGTGAAGAGCGGA | XhoI | Km |
| GATAAAAAGCTGGGTACCAGGATGCACCTTGAA | KpnI | ||
| CGACCACCGAGGCATGGTACCCAGCTTTTTATC | KpnI | ||
| CGGAGATCTCCGTCGGCACCACACAGCTC | BglII | ||
| csgA | TCACTCGAGGGATCAAAACTATTGTCCGT | XhoI | Km |
| AATGCTCAGGTACCGCCGTTATGATTACC | KpnI | ||
| ATAACGGCGGTACCTGAGCATTTATCAGT | KpnI | ||
| CGCAGATCTTAGCGCAGACGCTAAATTAA | BglII | ||
| metF | CGTCTCGAGGACATGAAGAAAATTCAACT | XhoI | Km |
| TTATTCCAGGTACCGCTCTTTGATGCCTT | KpnI | ||
| CAAAGAGCGGTACCTGGAATAACGGTATC | KpnI | ||
| TCCAGATCTTGGCAAATGGCATAACTCAT | BglII | ||
| ttrB | TCACTCGACCGCTGATTCTCTGGAGGA | XhoI | Km |
| CTTGTACCGGGTACCCCGGCACAC AGG | KpnI | ||
| GTCCCTGGATGCCTGGGTACCGGCCATAGG | KpnI | ||
| CGCAGATCTTGGCAATGTGGACGGGAG | BglII | ||
| ttrS | TCACTCGACCCCGGCTTGTTGTTGATC | XhoI | Km |
| ACTGGGCCGGGTACCCGTCCACCAGTC | KpnI | ||
| CCGCCTGAGCCGCATGGTACCCGCCCCAGT | KpnI | ||
| GCGAGATCTTCATCCAGTAGATGAAT | BglII | ||
| pduA | TCACTCGACCCATGCGAGGTCTTTATG | XhoI | Km |
| CGCGGCGATGTTACCCGGTCAAAG | KpnI | ||
| TGCATCGGTGGCCGCGGTAACATCGCCGCG | KpnI | ||
| CGCAGATCTCCACCAGCTGACTGCTGC | BglII | ||
| eutR | TCACTCGACGAGAGCCTCCCCATCAAT | XhoI | Km |
| GTGGCCAGCGTTACCTGCACAAAGCCC | KpnI | ||
| CTAGCGCTGGAGGTAGTTACCGCTGGCGAG | KpnI | ||
| CGGAGATCTGTCGGAGGGCCGGGCGTC | BglII | ||
| btuB | TCACTCGACAAGCCTGCGGCATCCTCC | XhoI | Km |
| CTCCGCTATGGTACCTTCCGATGCTAT | KpnI | ||
| GCGCTTTGTAGGAGGGGTACCATAGCGGAG | KpnI | ||
| CGGAGATCTCGGTGGGACGAGGTTCAG | BglII | ||
| cobS | GATCTAGAACGAATCTGCTGTTTGCGCT | XhoI | Km |
| CAGCAGGGTACCTAGCGGAATACCACACCAG | KpnI | ||
| CCGCTAGGTACCCTGCTGACCGGTGGTTTTCA | KpnI | ||
| AGTCTAGAACAGAGCCAGCAGAAAGATC | BglII | ||
| CAGCAGGGATCCTAGCGGAATACCACACCAGG | BamH1 | Spc | |
| CCGCTAGGATCCCTGCTGACCGGTGGTTTTCA | BamH1 | ||
| cbiA | CATCTAGAAAGGCATCACGCATTTATTC | XhoI | Km |
| CGTTATGGTACCAATGGCATTTTTGAGGAGCT | KpnI | ||
| GCCATTGGTACCATACGGTGATGTTAAAACAT | KpnI | ||
| TGTCTAGACAGCCAGTGCTGCACCATTT | BglII | ||
| TAACATCACCGGATCCGCCGCCAG | BamH1 | Spc | |
| AGCAATCATGGCATGGGATCCGGTGATGTT | BamH1 |
a
Underlining and boldface indicate enzyme sites.