Fig. 3.

Intranucleosome cross-linking has little effect on SWI/SNF-dependent exposure of restriction enzyme sites within the 5S nucleosomes. (A) Nucleosomes were reconstituted on 244-bp and 215-bp 5S DNA with H2A G2C and wild-type H3/H4, and the N-terminal tails within the 215-bp nucleosomes were cross-linked as described in the text. (B) The same amounts of cross-linked and un-cross-linked nucleosomes were mixed together and subjected to EcoRV digestion in the absence of SWI/SNF or presence of 1 nM SWI/SNF for the indicated times. (C) Plot of the fraction of uncut 5S nucleosomal DNAs versus digestion time.