Fig. 3.

Spt6 does not affect nucleosome positioning or occupancy over heterochromatin. (A) Bulk analysis of nucleosomes was conducted by partial MNase digestion and electrophoresis on an ethidium-stained agarose gel (left), followed by Southern blotting for the endogenous dg repeats (right). (B) The number of sites with changes in nucleosome occupancy in the clr4Δ and spt6-1 mutants, using 2.0 and 1.0 cutoffs for significance. (C) Nucleosome occupancy over three clr4Δ NFRs in wild-type (WT), spt6-1, and clr4Δ strains, displayed as the moving average of normalized log₂ scores over 19 probes. The site of overlap from panel B is depicted at left, with the clr4Δ and spt6-1 mutants showing opposing effects. The other clr4Δ NFRs showed minimal (center) or no (right) change in the spt6-1 mutant.