Fig. 5.

Measurement of histone H3 lysine 14 acetylation levels over the pericentric repeats in wild-type (WT) and spt6-1 strains. (A) ChIP analysis was performed to measure the level of H3K14 acetylation over the pericentric dg and dh repeats using antisera specific to H3K14ac and unmodified H3. In all cases, binding was assessed by quantitative real-time PCR. Columns represent the mean normalized value ± standard error (SE) (n = 6). Unnormalized data and no-antiserum controls are shown in Fig. S2, available at http://genepath.med.harvard.edu/∼winston/supplemental.htm. (B) Recruitment of the SHREC deacetylase complex to the pericentric repeats was measured by ChIP analysis of the subunit Clr3, which was C-terminally Flag tagged at its endogenous locus. Columns represent the mean normalized value ± SE (n = 6). Untagged controls indicate background levels of Clr3 binding. (C) Isogenic strains containing specific combinations of spt6-1 with gcn5Δ and mst2Δ were constructed by cross and tetrad analysis. Expression of the dg and dh transcripts were assessed by qPCR. Columns represent the mean normalized value ± SE (n = 3 to 4).