Table 2.
Mouse plasma and tissue lipid analysis by LC-MS/MSa
| Compartment and lipid | Chow diet |
High-fat diet |
||
|---|---|---|---|---|
| WT | Sptlc2+/− | WT | Sptlc2+/− | |
| Plasma | ||||
| Sph (ng/ml) | 30 ± 6 | 33 ± 7 | 41 ± 11 | 45 ± 10 |
| S1P (ng/ml) | 262 ± 39 | 227 ± 21 | 401 ± 36 | 293 ± 27* |
| Sa (ng/ml) | 36 ± 8 | 23 ± 8 | 26 ± 5 | 24 ± 3 |
| Sa1P (ng/ml) | 111 ± 16 | 86 ± 19 | 205 ± 57 | 105 ± 21* |
| DAG (ng/ml) | 1,508 ± 146 | 1,346 ± 119 | 8,129 ± 1393 | 7,250 ± 1480 |
| SM (nmol/ml) | 117 ± 26 | 96 ± 15 | 203 ± 16 | 175 ± 12* |
| PC (nmol/ml) | 1,752 ± 254 | 1,405 ± 383 | 5,289 ± 655 | 4,713 ± 383 |
| Cer (ng/ml) | 967 ± 99 | 813 ± 78 | 3,235 ± 381 | 1,569 ± 233* |
| DHCer (ng/ml) | 130 ± 26 | 105 ± 20 | 440 ± 50 | 219 ± 28* |
| GlyCer (ng/ml) | 5,307 ± 1212 | 3,354 ± 679 | 23,450 ± 5,210 | 19,569 ± 2,135 |
| GM3 (ng/ml) | 212 ± 29 | 182 ± 30 | 397 ± 51 | 366 ± 36 |
| Liver | ||||
| Sph (ng/mg) | 2.09 ± 0.31 | 1.96 ± 0.33 | 4.05 ± 0.61 | 3.59 ± 0.41 |
| Sa (ng/mg) | 0.42 ± 0.02 | 0.34 ± 0.08 | 1.17 ± 0.03 | 1.09 ± 0.05 |
| DAG (ng/mg) | 553 ± 78 | 486 ± 81 | 1351 ± 395 | 1297 ± 379 |
| SM (nmol/mg) | 0.74 ± 0.16 | 0.66 ± 0.15 | 1.10 ± 0.14 | 1.19 ± 0.05 |
| PC (nmol/mg) | 18.12 ± 6.60 | 19.51 ± 3.08 | 22.42 ± 1.50 | 23.59 ± 0.86 |
| Cer (ng/mg) | 133.7 ± 21.2 | 154.8 ± 18.3* | 143.7 ± 12.3 | 185.5 ± 15.7* |
| DHCer (ng/mg) | 17.4 ± 2.4 | 21.1 ± 3.5 | 17.8 ± 3.3 | 20.3 ± 1.7 |
| GlyCer (ng/mg) | 105.0 ± 19.3 | 82.9 ± 22.2 | 77.9 ± 15.6 | 79.7 ± 16.3 |
| GM3 (ng/mg) | 10.9 ± 1.3 | 9.5 ± 1.2 | 7.1 ± 1.1 | 7.9 ± 0.8 |
| Adipose tissue | ||||
| Sph (ng/mg) | 0.07 ± 0.01 | 0.09 ± 0.01 | 0.12 ± 0.00 | 0.09 ± 0.00 |
| Sa (ng/mg) | 0.14 ± 0.00 | 0.12 ± 0.01 | 0.15 ± 0.01 | 0.15 ± 0.01 |
| SM (nmol/mg) | 0.32 ± 0.03 | 0.36 ± 0.04 | 0.28 ± 0.05 | 0.32 ± 0.06 |
| PC (nmol/mg) | 1.09 ± 0.10 | 1.21 ± 0.29 | 1.11 ± 0.31 | 1.01 ± 0.21 |
| Cer (ng/mg) | 8.96 ± 0.77 | 8.77 ± 0.81 | 9.45 ± 1.10 | 8.88 ± 0.91 |
| GlyCer (ng/mg) | 0.70 ± 0.06 | 1.42 ± 0.31* | 3.27 ± 0.41 | 2.95 ± 0.36 |
| GM3 (ng/mg) | 0.37 ± 0.09 | 0.58 ± 0.11 | 0.40 ± 0.06 | 0.30 ± 0.10 |
| Muscle | ||||
| Sph (ng/mg) | 0.10 ± 0.02 | 0.09 ± 0.01 | 0.14 ± 0.04 | 0.11 ± 0.03 |
| Sa (ng/mg) | 0.11 ± 0.03 | 0.10 ± 0.01 | 0.13 ± 0.02 | 0.12 ± 0.02 |
| SM (nmol/mg) | 0.32 ± 0.08 | 0.28 ± 0.05 | 0.31 ± 0.08 | 0.35 ± 0.06 |
| PC (nmol/mg) | 8.03 ± 1.15 | 7.84 ± 1.66 | 5.59 ± 1.12 | 7.30 ± 1.75 |
| Cer (ng/mg) | 25.41 ± 4.33 | 25.11 ± 4.91 | 28.50 ± 3.82 | 27.75 ± 2.30 |
| GlyCer (ng/mg) | 46.51 ± 7.11 | 38.33 ± 6.91 | 39.10 ± 5.59 | 48.39 ± 6.99 |
| GM3 (ng/mg) | 10.60 ± 2.13 | 9.93 ± 1.48 | 9.05 ± 1.59 | 8.46 ± 1.36 |
a
Values are means ± SD (n = 5). *, P < 0.05 compared to WT. Sph, sphingosine; S1P, sphingosine-1-phosphate; Sa, sphinganine; Sa1P, sphinganine-1-phosphate; DAG, diacylglycerol; Cer, ceramide; DHCer, dihydroceramide; Glycer, glucosylceramide.