Fig. 9.

HuR-enhanced cell proliferation and survival depend partly on upregulation of nucleolin. HeLa cells were transfected with Ctrl siRNA, HuR siRNA, or miR-494 together with either a control plasmid (pEGFP) or with a plasmid expressing the nucleolin CR [pEGFP-NCL(CR)], and 48 h later, cells were analyzed. (A) The cells remaining in each transfection group were counted using a hemacytometer. (B) Whole-cell lysates prepared from each transfection group were analyzed by Western blotting to assess the levels of ectopic (Ect.) nucleolin, endogenous (Endog.) nucleolin, HuR, loading control β- actin, and cleaved PARP, an apoptotic marker. The data shown are means and standard deviations (A) or representative results (B) from three independent experiments.