Table 2.
Oligonucleotide primers for M-PCR for species identification of human-associated staphylococci
| primer | Sequence (5′–3′) | Size of PCR product (bp) | Species | Reference |
|---|---|---|---|---|
| hom-F | TACAGGGCCATTTAAAGACG | 177 | S. hominis | This study |
| hom-R | GTTTCTGGTGTATCAACACC | |||
| epi-F | TTGTAAACCATTCTGGACCG | 251 | S. epidermidis | This study |
| epi-R | ATGCGTGAGATACTTCTTCG | |||
| aur-F | TCGCTTGCTATGATTGTGG | 359 | S. aureus | 35 |
| aur-R | GCCAATGTTCTACCATAGC | |||
| hae-F | TAGTGGTAGGCGTATTAGCC | 434 | S. haemolyticus | This study |
| hae-R | ACGATATTTGCCATTCGGTG | |||
| cap-F | ACTACGCCTATGATTATTGC | 525 | S. capitis | This study |
| cap-R | GAYGCTTCTTTACCATAGGG | |||
| lug-F | TCCAATGATGGTAACGAGGC | 695 | S. lugdunensis | This study |
| lug-R | TTTTGCGCCTCGTTTTGTGC | |||
| sap-F | TTTTGGATGCGATAGATTGG | 843 | S. saprophyticus | This study |
| sap-R | TCTTCAGACTTTTCAAAGGC | |||
| war-F | CGTTTGTAGCAAAACAGGGC | 999 | S. warneri | This study |
| war-R | GCAACGAGTAACCTTGCCAC | |||
| rae-F | TTGTTCTWGCACTYATTGCG | 1,227 | S. caprae | This study |
| rae-R | TTTTATAGAACAGGGTCGAC |