(A) Detection limit and real-time reaction of the H. influenzae type b LAMP assay, as monitored by real-time measurements of turbidity. Shown from left to right are the curves of decreasing bacterial concentrations, from 1,000,000 to one genome copy. (B) The relationship between the threshold times (Tt) of each sample and the log of the amount of initial template DNA. The linear determination range was from 10 to 1,000,000 microorganisms per reaction mixture.