Table 1.
Characteristics of 46 H. influenzae strains
| H. influenzae (no. of strains)a | No. of strains | Origin of isolateb | Capsule type(s)c | Hib agglutination testd |
Hib LAMP testinge | |
|---|---|---|---|---|---|---|
| Slide | Latex | |||||
| Standard strains (8)* | 5 | — | a, c, d, e, f | – | – | – |
| 1 | — | b | + | + | + | |
| 2 | — | NT | – | – | – | |
| Reference strains (18)† | 2 | RT | a, f | – | – | – |
| 1 | CSF | b | – | + | + | |
| 7 | CSF | b | + | + | + | |
| 2 | CSF | c, f | – | – | – | |
| 5 | RT | NT | – | – | – | |
| 1 | RT | NT | + | – | – | |
| Clinical strains (20)‡ | 8 | N | b | + | + | + |
| 1 | N | b | – | + | + | |
| 11 | N | NT | – | – | – | |
*, Obtained from the Institute of Medical Science, University of Tokyo, Tokyo, Japan; †, provided by Mogens Kilian, Institute of Medical Microbiology and Immunology, Aarhus University, Aarhus, Denmark; ‡, obtained from the Department of Microbiology, Gifu University School of Medicine, Gifu, Japan.
RT, respiratory tract specimen; CSF, cerebrospinal fluid; N, nasopharyngeal swab.
Capsule types were confirmed by PCR (5). NT, nontypeable.
+, Positive; –, negative. Slide agglutination serotyping was performed with H. influenzae antisera specific for type b. Latex agglutination serotyping was performed for the detection of H. influenzae serotype b.
+, Amplification after a 35-min incubation; –, no amplification after a 60-min incubation.