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Normalized melting peaks between 58.12°C and 73°C showing a nonspecific mutant (T816C), the wild type, and the H275Y mutant at 61.5°C, 65.25°C, and 69°C, respectively. The nonspecific mutant (two mismatches) is clearly differentiated from the wild type (single mismatch) and the H275Y mutant (perfect match).
