Table 1.

Comparison of mutant–wild-type mixture detection and quantification using in-house Sanger sequencing, HRM, and pyrosequencing methods

Sample no.	Sanger sequencing	HRM (% mutant [range])	Pyrosequencing (% mutant)	CT value
1	Mixture	Mixture (10–20)	Mixture (21.2)	26.2
2	Mixture	Mixture (80–90)	Mixture (67.8)	27.0
3	Mixture	Mixture (60–70)	Mixture (68.9)	25.9
4	Mutant	Mixture (90–100)	Mixture (86.2)	21.8
5	Not amplified	Mixture (50–60)	Wild type	34.6
6	Mixture	Mixture (80–90)	Mixture (70.8)	29.0
7	Not amplified	Mixture (70–80)	Mixture (13.7)	33.6