Table 1.

Sequences and molecular weights of unextended (UEP) and extended primers to detect 15 high-risk HPV types in a single multiplex reaction

HPV type	Forward primera/competitora,c	Reverse primera	Extension primera	No. of basesb; or Dad
16	ACGTTGGATGAACAGTTACTGCGACGTGAG/AGCATATGGATTCCCATCTCTATATACTATCCATAAATCCCGAAAAGCAAAGTCATATACCTCACGTCGCAGTAACTGTT	ACGTTGGATGAGCATATGGATTCCCATCTC	TTGCTTTTCGGGATTTATG	73; 5,831
18	ACGTTGGATGATAGCTGGGCACTATAGAGG/TGTGTTTCTCTGCGTCGTTGGAGTCGTTCCTGTCGTGCTCCGTTGCAGCACGAATGGCACTGGCCTCTATAGTGCCCAGCTAT	ACGTTGGATGTGTGTTTCTCTGCGTCGTTG	GCCATTCGTGCTGCAAC	83; 5,146
31	ACGTTGGATGTGGTGAACCGAAAACGGTTG/CAGGATTTTTGAACATGGCGTCTGTAGGTTTCCACAAAATACTATGTGCTTTATATACCAACCGTTTTCGGTTCACCA	ACGTTGGATGCAGGATTTTTGAACATGGCG	ATGGCGTCTGTAGGTTT	78; 5,247
33	ACGTTGGATGCGATTTCATAATATTTCGGG/TCACAGTGCAGTTTCTCTACGTCGGGACCTCCAACACGCCGCACAGCGCCCTCCCCAACGACCCGAAATATTATGAAATCG	ACGTTGGATGCACAGTGCAGTTTCTCTACG	ACACGCCGCACAGCGCCCT	81; 5,704
35	ACGTTGGATGCGCTGTGTCCAGTTGAAAAG/TTCCAACAGGACATACACCGACCTGTCCACCGTCCACGGATGTTATGGAATCGTTTTTTTTCTTCTAAATGTCTTTGCTTTTCAACTGGACACAGCG	ACGTTGGATGTTCCAACAGGACATACACCG	ACCTGTCCACCGTCCAC	97; 5,051
39	ACGTTGGATGGCCATACAAATTGCCAGACC/TCGTCTGCAATAGACACAGGCTATTGTAATGTCCTGCAAGGTGGTGTCCAGGGTTGTGCACAGGTCTGGCAATTTGTATGGC	ACGTTGGATGTCGTCTGCAATAGACACAGG	TTGCCAGACCTGTGCACAAC	82; 6,062
45	ACGTTGGATGAAGTGCATTACAGGATGGCG/CTGTGCACAAATCTGGTAGCTTGTAGGGTCGTTCCTTTGGATCGTCAAAGCGCGCCATCCTGTAATGCACTT	ACGTTGGATGCTGTGCACAAATCTGGTAGC	AAATCTGGTAGCTTGTAGGGTCGTT	72; 7,743
51	ACGTTGGATGGGTTATGACCGAAAACGGTG/GTCTTTCCCTCTTGTCTTCGAACATGGTGTTCTTCTATACTTTTAGCACTGCACTTTTATATGCACCGTTTTCGGTCATAACC	ACGTTGGATGGTCTTTCCCTCTTGTCTTCG	CGGTGCATATAAAAGTGCAGTG	83; 6,824
52	ACGTTGGATGATTGTGTGAGGTGCTGGAAG/ACCTCTCTTCGTTGTAGCTCTTTTTTGCACTGCACACACTGCAGCCTTATTTCATCCACCGATTCTTCCAGCACCTCACACAAT	ACGTTGGATGACCTCTCTTCGTTGTAGCTC	GTGCTGGAAGAATCGGTG	84; 5,620
56	ACGTTGGATGGTTAACTCCGGAGGAAAAGC/CAAACATGACCCGGTCCAACCATGTGCTATTAGATGAAATGGTCTTTTTCTGTCACAATGCAATTGCTTTTCCTCCGGAGTTAAC	ACGTTGGATGCAAACATGACCCGGTCCAAC	CAACCATGTGCTATTAGATGAAAT	85; 7,360
58	ACGTTGGATGTGATTTGTGTCAGGCGTTGG/TACCTCAGATCGCTGCAAAGTCTTTTTGCATTCAACGCATTTCAATTCGATTTCATGCACACATGTCTCCAACGCCTGACACAAATCA	ACGTTGGATGTACCTCAGATCGCTGCAAAG	GTGTCAGGCGTTGGAGACAT	88; 6,213
59	ACGTTGGATGCTGCCTGATTTGAGCACAAC/GCAGTTCCCCTTTGCAAAACACACAATTGATGGGAATATCATGCAGAGGAATATTCAATGTTGTGCTCAAATCAGGCAG	ACGTTGGATGGCAGTTCCCCTTTGCAAAAC	TTGCAAAACACACAATTGATG	79; 6,422
66	ACGTTGGATGCGGAGGAAAAACAATTGCAC/CCGGTCCATGCATATGCTATATAATGAAATCGTCTTTTATATTCACAGTGCAATTGTTTTTCCTCCG	ACGTTGGATGCCGGTCCATGCATATGCTAT	AGGAAAAACAATTGCACTGTGAA	87; 7,113.7
68	ACGTTGGATGTGCAGAAGGCAACTACAACG/ACCCCGTCCCTATATACTACATTTAAGTCAGCAAAGGCAAATTCATATACCTCTGTCCGTTGTAGTTGCCTTCTGCA	ACGTTGGATGACCCCGTCCCTATATACTAC	CCCTATATACTACATTTAAGTCA	77; 6,942
73	ACGTTGGATGTGTACAGCGTCCGGTCCAC/TGTACAGCGTCCGGTCCACTGTTCTCCTATTTGATGAAACCGTTTTTTTTCATCTACATGCTTTTGCTTTTCCAGTGGA	ACGTTGGATGTCCACTGGAAAAGCAAAAGC	GTCCGGTCCACTGTTCT	99; 5,128.3

^aAll primers are listed 5′ - 3′. The sequence of the competitor primer for each HPV type is shown in a separate row immediately below the corresponding set of forward, reverse, and extension primers.

^bLength of HPV type-specific PCR products amplified with forward and reverse primers.

^cBold and underlined C, G, or A indicates the base of the competitor sequence that is the complement to the wild-type HPV type-specific sequence.

^dMass in Da of extension primers.