Table 1.
Comparison of the results of VI and rRT-PCR from testing sequential semen samples collected from a carrier stallion
| Semen collection date (mo/day/yr) | Semen sample identification no. |
CT values obtained with combination ofa: |
Virus isolation |
||||
|---|---|---|---|---|---|---|---|
| MagMAX kit (ABI) |
BioSprint kit (Qiagen) |
Virus isolated | Virus titer (PFU/ml) for high-passage RK-13 cells (passage number) | ||||
| QuantiFast kit (Qiagen) (C1) | TaqMan EZ kit (ABI) (C2) | QuantiFast kit (Qiagen) (C5) | TaqMan EZ kit (ABI) (C6) | ||||
| 01/09/2008 | S-4168 | 33.16 | − | − | − | + | 1 × 101 (P404) |
| 01/15/2008 | S-4178 | 31.40 | − | − | − | + | 5 × 101 (P405) |
| 01/28/2008 | S-4256 | 38.25 | 33.37 | 35.50 | 35.42 | − | − |
| 02/08/2008 | S-4257 | 37.83 | 34.60 | 37.14 | 35.33 | − | − |
| 02/11/2008 | S-4258 | 36.25 | 32.98 | 34.93 | 36.57 | − | − |
| 02/13/2008 | S-4259 | 35.34 | − | 38.07 | − | + | 1.5 × 101 (P402) |
| 02/20/2008 | S-4260 | 33.57 | 37.35 | 34.91 | − | + | 7.5 × 101 (P401) |
a
CT, cycle threshold; −, negative.