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Broad-range PCR assay. The band corresponding to the 330-bp sequence of 16S rRNA gene of the genus Clostridium is indicated (arrow). Lane 1, 100-bp ladder; lane 2, pericardial isolate; lane 3, C. sordellii laboratory isolate; lane 4, C. difficile ATCC 9689; lane 5, C. perfringens laboratory isolate; lane 6, negative control.
