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. 2011 Oct;31(19):3963–3974. doi: 10.1128/MCB.05297-11

Fig. 2.

Fig. 2.

Requirements for transcriptional activation by Tim-3. Jurkat T cells (A to C) were transfected with an NFAT/AP-1 (A and B) or NF-κB (C) reporter plus the indicated Tim-3 constructs. D10 T cells (D) were also transfected with an NFAT/AP-1 reporter and the indicated Tim-3 constructs. Jurkat T-cell lines lacking expression of either ZAP-70 (E) or SLP-76 (F) were transfected with NFAT/AP-1-luciferase and the indicated constructs. The next day, cells were left unstimulated (white bars) or stimulated with anti-TCR/CD28 monoclonal antibodies (MAbs) (black bars). Luciferase activity is presented as the percentage of maximal stimulation obtained with PMA-ionomycin. Luciferase activity is presented as the percentage of the maximal response obtained with PMA plus ionomycin. Results shown are the average ± SD of data for triplicate samples from a single experiment, representative of at least three that were performed in each case.