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Decreased expression of DNMT1 by TSA treatment. Hep3B cells were treated with TSA for 24 h. Cytoplasmic and nuclear proteins were resolved by SDS-PAGE, and DNMT1 was detected by Western blotting. Nuclear DNMT1 protein levels show a dose-dependent decrease. The graph shows the average of triplicate experiments, with error bars denoting standard errors of the means. Significance was calculated with a Student's t test. **, P < 0.01; ***, P < 0.001.
