Fig. 3.
Decreased global DNA methylation by TSA treatment. Hep3B cells were treated by TSA for 24 h, and genomic DNA was extracted and analyzed for DNA methylation by LUMA (A) and NNA (B). Bars represent mean values ± standard deviations (triplicate experiments). Both assays revealed significant hypomethylation following TSA treatment. (C) Induction of p21waf1 by TSA treatment. Hep3B cells were treated by TSA for 24 h and then harvested and analyzed by Western blotting. P values were calculated using a Student's t test. **, P = 0.004; ***, P = 0.001.