Fig. 2.

Effect of Rsp on MW2 biofilm formation. (A) Biofilm formation was assayed in 96-well microtiter plates for MW2 (pML100) and MW2 Δrsp mutant with pML100 or pML3871 (i.e., pML100-rsp). Biofilm medium was supplemented with 5 μg per ml of chloramphenicol. The graph represents the average absorbance of the eluted crystal violet-stained wells from 3 independent experiments. A representative well stained with crystal violet of each strain is shown below the graph. (B) Biofilm formation in flow cells. Selected pictures taken every 20 min are shown with specific time points indicated.