Fig. 3.

Northern blot analysis of rsp transcription. (A) Total RNAs were prepared from MW2 cultures grown to different OD₆₆₀s as indicated above the figure. Total RNA of 5 μg from each sample was applied to the formaldehyde gel and hybridized with a specific rsp (751-bp) probe. Ethidium bromide-stained ribosome bands are shown below as the loading control. (B) Lane 1, 5 μg of total RNA prepared from MW2 with rRNA removed. Lane 2, 3 μg of total RNA from MW2. Lane 3, total RNA of 3 μg from MW2 Δrsp. Cultures were grown to an OD₆₆₀ of 1.0.