Table 2.
Oligonucleotide primers used for plasmid and strain construction
| Primer purpose and name | Sequence |
|---|---|
| Plasmid and strain construction | |
| MW2301-1 | GGGGACAAGTTTGTACAAAAAAGCAGGCTGCGAATATTTTGTAACAGGGCTA |
| MW2301-2 | ATCTTTACGACTCGCGGCCGCTTCGATTATATTTTCGCCGTGGTT |
| MW2301-3 | GAAAATATAATCGAAGCGGCCGCGAGTCGTAAAGATAATAACTACCA |
| MW2301-4 | GGGGACCACTTTGTACAAGAAAGCTGGGTTCGATTTTGATAGGTGTATCTAATGA |
| MW2301-8 | GAATTCCACAAGAGGACTGTTAGTATTTAGC |
| MW2301-12 | GGATCCCCATGTTTTACATTTAAATGATAGATTTC |
| MW2301-14 | AGATCGAAGTCAGCGTGCCAAAGT |
| MW2301-15 | AGCTAATGCCACCGCCATATCGTA |
| FnbA5′1 | ACAAGGGTTTATGATGACTTGAATACAATTTATAGG |
| FnbA5′2 | CGTACCTAAGATTGTTTTTCACTATAATATCTCCC |
| FnbA-1 | GGGGACAAGTTTGTACAAAAAAGCAGGCTGGTGCAGGTAGTGTGATTTTG |
| FnbA-2 | TCCGCCGAACAACATAGCGGCCGCCCGCTATTGTCTTGAGATTGTGTT |
| FnbA-3 | TCAAGACAATAGCGGGCGGCCGCTATGTTGTTCGGCGGATTATTCAGC |
| FnbA-4 | GGGGACCACTTTGTACAAGAAAGCTGGGTGATGGTTGCTCAGTTGATGTC |
| FnbA-6F | GTCGACGCATTTAAAGGGAGATATTATAGTGAAAAACAATCTTAGG |
| FnbA-7R | GAGCTCCAATGAAGCAATCAGAAAACACTCT |
| FnbB-1 | GGGGACAAGTTTGTACAAAAAAGCAGGCTTATGTTGTTCGGCGGATTATTCAGC |
| FnbB-2 | TCCGAACAGTATACCGCGGCCGCGATGGTTGCTCAGTTGATGTC |
| FnbB-3 | AACTGAGCAACCATCGCGGCCGCGGTATACTGTTCGGAGGATTG |
| FnbB-4 | GGGGACCACTTTGTACAAGAAAGCTGGGTCATACGTTCAATCGCATCTGTT |
| FnbB-6F | GTCGACTACATTTAAGGGAGAATATTATAGTGAAAAG |
| FnbB-7R | GAATTCGAACGCCTTCATAGTGTCATTAAGTATGTA |
| sasG-1 | GGGGACAAGTTTGTACAAAAAAGCAGGCTGTGTTTATAGCTAGCACCACAAACT |
| sasG-2 | CATTAATCCAGCAATTCCAATTATACTGTGAGCCAATTAAAATAGATGCTG |
| sasG-3 | CAGCATCTATTTTAATTGGCTCACAGTATAATTGGAATTGCTGGATTAATG |
| sasG-4 | GGGGACCACTTTGTACAAGAAAGCTGGGTCTATCGATTAACGCATTACTTGTTGCT |
| scpA-1 | GGGGACAAGTTTGTACAAAAAAGCAGGCTTCCACTTCTAACCATGCTTCATAG |
| scpA-2 | GATGAATACCATTGATAATGGTCGCCATTGCTCAGCGTTTGCAATAGGGGTAACACT |
| scpA-3 | AGTGTTACCCCTATTGCAAACGCTGAGCAATGGCGACCATTATCAATGGTATTCATC |
| scpA-4 | GGGGACCACTTTGTACAAGAAAGCTGGGTCTGGACTAAAGGCATTCGTCGTAG |
| spa-6 | TCTAGAGGATCCATACCCCCTGTATGTATTTGT |
| spa-7 | CCCGGGGATCCAAACAATACACAACGATAGA |
| spa-11 | GGGGACAAGTTTGTACAAAAAAGCAGGCTCATCAGCAAGAAAACACACT |
| spa-12 | GGGGACCACTTTGTACAAGAAAGCTGGGTCTGCCTTTGTATTAGTATGG |
| Rsp1 | GGGGACAAGTTTGTACAAAAAAGCAGGCTTGTTCATAGACTGTGTAAACTATATTC |
| Rsp2 | GGGGACCACTTTGTACAAGAAAGCTGGGTTTATTAAATCTTGGAAACGAGTGAACGGAAC |
| Real-time RT-PCR | |
| SGfnbAF2 | GAATATTTGCAAGGGTCAGATCAGGTTAATTTTAGAACTG |
| SGfnbAR2 | CTGTGTGGTAATCAATGTCAAGCGGTGTATTG |
| fnbBF1 | GTAGAGGAAAGTGGGAGTTCAG |
| fnbBR1 | TGTGTTGATTGTGATGGTTGC |
| SGhlb1 | AAACACCTGTACTCGGCCGTTCTCAATCAG |
| SGhlb2 | ACTTACAATCGCTACGCCACCATCTTCTGC |
| ureA1 | TTGCACGTCGTGGTAAAGCA |
| ureA2 | AACTCTGCAACGGTCTTACC |
| SGmap1 | CCGCAGCTAAGCCATTAGA |
| SGmap2 | AGCGTTCACAGTGATTGCATA |
| spaF2 | GCCTTAAAGACGATCCTTCAGTGAGCAAAG |
| spaR2 | CCAGGTTTAACGACATGTACTCCGTTGC |
| SGgyrB3 | GGAATCGGTGGCGACTTTGATCTAGCGAAA |
| SGgyrB4 | CGCTCCATCCACATCGGCATCAGTCATAAT |