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Cells harboring UmuC variants N32A, N33A, and D34A are sensitive to 4-NQO (A), while only cells harboring UmuC variant N33A are sensitive to NFZ (B). (A and B) Assays were performed with the pGY9738 plasmid and the following derivatives in AB1157: pGB2 (empty vector; ◆), pGY9738 (umuD′C wild type; ■), pGY9738-N32A (umuD′C N32A; ●), pGY9738-N33A (umuD′C N33A; ♢), and pGY9738-D34A (umuD′C D34A; ○).
