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pH profile of GlmA activity and stability. GlmA was incubated in various buffers at different pH and activity was determined with GlcN and [acetyl-1-14C]CoA by applied TLC as described in Materials and Methods. The buffers used were phosphate-citrate at pH 4.5 to 8.0, Tris-HCl at pH 7.5 to 9.0, and glycine-NaOH at pH 9.0 to 10.5. The data are means of four independent experiments; errors were <5%.
