Fig. 6.
GlmA acetylates terminal GlcN of non-N-acetylated muropeptides prepared from B. subtilis cell wall. Muropeptides were generated by mutanolysin digestion of the cell wall peptidoglycan that could be acetylated, using [acetyl-1-14C]CoA, by GlmA at GlcN residues of N-deacetylated peptidoglycan. After treatment with mutanolysin and AmiD, an acetylated disaccharide product can be obtained upon GlmA acetylation. The radioactively labeled GlcNAc of this product could be released after treatment with NagZBs, which was added subsequent to the acetylation reaction. Lanes 1 to 3, standards as indicated. AcCoA, acetyl-CoA.