Table 1.
Substrate | Vmax (μmol/min/mg) | Km (μM) | kcat (1/s) | kcat/Km (1/s/mM) |
---|---|---|---|---|
GlcN | 2.11 | 114.3 | 1.35 | 11.8 |
(GlcN)2 | 2.69 | 64.5 | 1.72 | 26.7 |
(GlcN)3 | 2.60 | 39.2 | 1.66 | 42.4 |
AcCoA | 2.10 | 46.4 | 1.34 | 28.9 |
Kinetic parameters were determined in 100 mM sodium phosphate (pH 7.27) at 30°C as described in Materials and Methods. Data represent the means of two independent experiments. Standard errors were <5%. AcCoA, acetyl-CoA.