Table 3.
Inhibition of antibody binding by native and de-O-acetylated PS
| Inhibition PS | Bindinga of indicated group 11 antiserum or MAb to target PS (antiserum, target PSb) |
||||||
|---|---|---|---|---|---|---|---|
| 11b, 11C | 11b, 11F | 11c, 11C | 11f, 11C | 11g, 11B | Hyp11AM2 11F | ||
| 11A | Native | − | − | + | − | − | + |
| deOAcc | − | − | − | − | − | − | |
| 11B | Native | + | + | − | + | + | − |
| deOAc | − | + | − | − | − | − | |
| 11C | Native | + | + | + | + | − | − |
| deOAc | − | + | − | − | − | − | |
| 11D | Native | − | + | + | − | − | + |
| deOAc | − | + | − | − | − | − | |
| 11E | Native | − | − | + | − | − | − |
| deOAc | − | − | − | − | − | − | |
| 11F | Native | − | + | − | − | + | + |
| deOAc | − | + | − | − | − | − | |
a
+ inhibition, PS is capable of competitively inhibiting antibody binding to target PS (≤50% signal compared to control); − inhibition, PS is unable to competitively inhibit antibody binding (>50% signal compared to control).
b
Native PS used to coat ELISA plates.
c
deOAc, PS de-O-acetylated by mild alkali hydrolysis.