Skip to main content
. 2011 Oct;193(19):5179–5190. doi: 10.1128/JB.05355-11

Fig. 4.

Fig. 4.

(A) Analysis of β-galactosidase activity of pDNlac-flgAp, pDNlac-flgBp, pDNlac-flgFp, pDNlac-fliEp, pDNlac-flhAp, and pDNlac-flrBp plasmids in A. hydrophila wild-type (AH-405) and the AH-3 flrA or AH-3 flrBC mutant after growth in TSB at 25°C. As a control we also measured the pDN19lacΩ promoterless plasmid. The results shown are representative of three independent experiments. (B) RT-PCR amplification of flgA, flgB, flgF, fliF, flhA, and flrB from cDNA of the AH-3 (lanes 1) strain and the AH-3 flrA (lanes 2) and AH-3 flrBC (lanes 3) mutants. A. hydrophila ribosomal 16S (rrsA) amplification was used as a control for cDNA template. RT-PCR amplifications were performed at least twice, with total RNA preparations obtained from a minimum of two independent extractions.