Fig. 2.

IolR-DNA-binding assay. Increasing concentrations of purified IolR-His₆ protein (indicated at the top of each lane) were incubated with 10 ng of 8 different PCR products. The positions of free DNA (open arrowheads) and IolR-DNA complexes (solid arrowheads) are indicated. As a control, 0.3 μM protein without DNA in binding buffer was loaded. (A) EMSA with the upstream regions of idhA, iolY, iolR, iolC, iolA, and nodD1 (IolR-independent control). (B) The identified IolR-binding motifs of the idhA, iolY, iolR, and iolC genes; controls 1 and 2, DNA fragments containing the sequences up- and downstream of the IolR-binding motif of iolY, respectively; lanes M, 100-bp ladder.