Fig. 3.
Transcriptional organization of S. meliloti inositol catabolic genes. The 2011 wild-type strain was grown in minimal medium with myo-inositol (MI) or glycerol (Gly) as the sole carbon source. The total RNA was purified and used as a template in RT-PCR (RT). cDNA was amplified with primers flanking the indicated intergenic regions of the iolYRCDEB cluster and the intragenic regions of the idhA, iolA, and smc01981 (cytochrome c [cC]) genes. Boxes represent the individual inositol genes, and arrows represent transcriptional units. As controls, PCR was performed with RNA samples (no RT) and no template (H2O).