Table 3.
Response of the proH-treA reporter fusion strain JSB7 in response to ionic and nonionic osmolytes
| Medium | TreA activitya (mean U mg of protein−1 ± SD) |
|---|---|
| SMM | 38 ± 2 |
| SMM + 0.4 M NaCl | 70 ± 4 |
| SMM + 0.4 M KCl | 74 ± 3 |
| SMM + 0.62 M sucrose | 208 ± 9 |
| SMM + 0.61 M lactose | 264 ± 9 |
| SMM + 0.68 M glycerol | 32 ± 2 |
a
The cultures were grown to early exponential growth phase (OD578 = 0.5 to 0.8), and their TreA enzyme activities were then assayed. The values given are from two independently grown cultures, and each TreA assay was performed twice. The osmolarity of the growth medium was raised in each culture to 1,100 mosmol kg of water−1 through the addition of salts, sugars, or glycerol.