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. 2011 Oct;193(19):5062–5072. doi: 10.1128/JB.05683-11

Fig. 4.

Fig. 4.

Examples of Tsr control cable mutants with ATT-mimic [1] signaling defects. (A) Output curves for Tsr(S217) mutants. These curves use the same shading convention for modification states and hosts as the curve for wild-type Tsr shown in Fig. 3A. y axis, extent of CW rotation time; x axis, dynamic range of the HAMP and MH bundles; vertical gray line, wild-type adaptation set point. The curves for different mutant receptors are stacked simply to conserve space. (B) Modification profiles of the S217 mutant receptors in various host strains. Unlabeled lanes are EEEE/QEQE/QQQQ markers (Fig. 3B). WT, wild type. (C) Modification profiles of the S217 mutant receptors in an adaptation-competent (CheR+ CheB+) host before (−) and after (+) experiencing a serine stimulus (SER). Unlabeled lanes are EEEE/QEQE/QQQQ markers (Fig. 3B).