Table 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s) or descriptiona | Reference or source |
|---|---|---|
| E. coli strains | ||
| DH5α | Cloning host | 16 |
| BL21(DE3) | Expression host | 49 |
| A. tumefaciens strains | ||
| C58 | Wild type | C. Baron, Montreal, Canada |
| C58 choX::Gm> | choX::Gm> insertion mutant of C58 | This study |
| C58 choX::Gm< | choX::Gm< insertion mutant of C58 | This study |
| C58 ΔpmtA | pmtA deletion mutant of C58 | 53 |
| C58 ΔpmtA choX::Gm> | pmtA choX::Gm> double mutant of C58 | This study |
| C58 ΔpmtA choX::Gm< | pmtA choX::Gm< double mutant of C58 | This study |
| C58 Δpcs | pcs deletion mutant of C58 | 53 |
| C58 Δpcs choX::Gm< | pcs choX::Gm< double mutant of C58 | This study |
| Plasmids | ||
| pBluescriptKS(+) | E. coli cloning vector, lacZ, Ampr | Stratagene, Santa Clara, CA |
| pAC01 | Transcriptional lacZ fusion vector containing promoterless lacZ gene; Tcr Apr | 26 |
| pET24b(+) | Expression vector, T7 promoter; 3′ His codon, Kmr | Novagen, Darmstadt, Germany |
| pVSBADNco | A. tumefaciens expression vector; Spr Smr | 55 |
| pK19mobscacB | Suicide plasmid; Kmr | 43 |
| pYP5 | pBSL15 derivative carrying Gm> | 18 |
| pBO893 | pVSBADNco carrying choX | This study |
| pBO2002 | pET24b carrying choX | This study |
| pBO2017 | pAC01 carrying a choX-lacZ fusion | This study |
| pBO890 | pAC01 carrying a choW-lacZ fusion | This study |
| pBO891 | pAC01 carrying an atu2278-lacZ fusion | This study |
| pBO2004 | pBluescript-choX::Gm> | This study |
| pBO2005 | pBluescript-choX::Gm< | This study |
| pBO2006 | pK19mobsacB-choX::Gm> | This study |
| pBO2007 | pK19mobsacB-choX::Gm< | This study |
a
Ap, ampicillin; Gm, gentamicin; Km, kanamycin; Sm, streptomycin; Sp, spectinomycin; Tc, tetracycline.