Fig. 2.

Transcription and translation of yidD. (A) RT-PCR analysis of E. coli RNA using AMV RT polymerase with the indicated primers showed that yidD gene is cotranscribed with yidC. gDNA, genomic DNA. (B) β-Galactosidase assay using MC4100-A harboring the reporter plasmid pTL61T-Nco-YidDprom showed that yidD mRNA is translated. As a negative control, MC4100-A harboring the empty pTL61T-Nco was used. The results are an average of three independent measurements. (C) MC4100-A cells containing pEH3His-YidD were grown in LB medium to an OD₆₆₀ of 0.3 and induced with 1 mM IPTG. Whole-cell samples were taken 1 h later and analyzed by SDS-PAGE and Western blotting using antisera against the His tag, YidC, and PspA. α, anti.