Fig. 4.

GFP-YidD and GFP-YidDH1 localize to the membrane. (A) Schematic representation of the constructs used. The boxes represent predicted α-helices while the lines represent coiled regions of YidD (Fig. 1C). The dashed line represents the linker region between GFP and YidD. (B) MC4100-A cells expressing GFP and GFP fusion proteins were grown to the early log phase of growth. Protein expression was induced with 50 μM IPTG for 1 h. Subsequently, the cells were collected, fixed, and imaged by fluorescence microscopy while micrographs of corresponding fields are shown by phase-contrast microscopy.