Table 1.
Strains, plasmids, and primers
| Strain, plasmid, or primer | Description or sequence | Reference or source |
|---|---|---|
| E. coli strains | ||
| Top10F′ | F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 nupG recA1 araD139 Δ(ara-leu)7697 galE15 galK16 rpsL(Strr) endA1 λ− | Invitrogen |
| MC4100-A | F− ΔlacU169 araD139 rpsL150 relA1 ptsF rbs flbB5301; arabinose resistant | 23 |
| MC4100(DE3) | MC4100 with λ DE3 | 53 |
| ΔyidD strain | yidD knockout in MC4100 (DE3) | This study |
| Plasmids | ||
| pKD13 | Template containing kanamycin cassette | 8 |
| pTL61T | lacZ ORF | 30 |
| pTL61T-Nco | NcoI site at the start of the lacZ ORF | This study |
| pTL61T-Nco YidDprom | yidD promoter sequence fused to lacZ ORF | This study |
| pEH3 | IPTG inducible expression plasmid | 22 |
| pEH3His-YidD | yidD ORF with 5′ His tag | This study |
| pEGFP | gfp ORF | Clontech |
| pEH3GFP-YidD | gfp ORF fused to 5′ of yidD ORF | This study |
| pEH3GFP-YidDH1 | gfp ORF fused to 5′ of yidD helix 1 ORF | This study |
| pEH3GFP | gfp ORF | This study |
| pCL-M13P2 | m13p2 ORF | 46 |
| pCL-CyoA-HA | cyoA ORF with 3′ HA tag | 45 |
| pC4Meth108FtsQCys15 | Sequence coding for 108 residues of FtsQ with a cysteine at position 15 | 55 |
| pC4Meth108FtsQCys36 | Sequence coding for 108 residues of FtsQ with a cysteine at position 36 | 55 |
| pC4Meth108FtsQCys61 | Sequence coding for 108 residues of FtsQ with a cysteine at position 61 | 55 |
| Primers | ||
| RT1 | TAGCCCTCATTCGGGTCTATC | |
| RT2 | TCTCTGGTATCAAATGGTC | |
| RT3 | ACCTCAGGCCCAACAGACCAC | |
| RT4 | ACGCCGTCGCGAAATAC | |
| RT5 | AGCCGGTACTGGTTCAGCCTG | |
| RT6 | TCTTCAGCTGCATCGGGTC | |
| pTL61T-NcoFw | CACACAGGAAACAGCCATGGCCATGATTACGGATTC | |
| pTL61T-NcoRv | GAATCCGTAATCATGGCCATGGCTGTTTCCTGTGTG | |
| YidDpromFw | GGCTATGTCGACCCTAAA CTGCGCAAGAGATC | |
| YidDpromRv | TTAGGCGCCATGGTTTTTCCAACGCTTCCG | |
| YidDpromminNcoFw | TTAATCACCCTTGGCTTCGG | |
| YidDpromminNcoRv | CCGAAGCCAAGGGTGATTAAAG | |
| YidDNterm-Xba-SD-His6Fw | TATCTAGATAGGAGGAGTTTTTACCATGCACCACCACCACCACCACGGATCTATGGCGCCGCCACTGTCG | |
| YidDCterm-SacIRv | TATAGAGCTCATTAGTGTTCTCTGGTATCAAATG | |
| GFP5′XbaFw | CCGGGTCTAGAAGAAGGAGATATACATATGAGTAAAGGAGAAGAAC | |
| YidD3′stopRv | TTCCGAGCTCTTATTAGTGTTCTCTGGTATC | |
| YidD3′NtermstopRv | TTCCGAGCTCTTATTAGAGTAGCGGACTAATCAGGCG | |
| GFP3′SacIRv | TTCCGAGCTCTTATTATTTGTATAGTTCATCCATG | |
| YidD_Ecoli_swap_H1 | GACTAAAAGATTGCGTTGCGAATCCATCGTTAGTGTTCTCTGGTATCAAAATTCCGGGGATCCGTCGACC | |
| YidD_Ecoli_swap_H2 | TTGGAAAAATTATGGCGCCGCCACTGTCGCCTGGCTCGCGGGTCCTGATATGTAGGCTGGAGCTGCTTCG |