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P74 cleavage does not happen in C-OBs produced in three cell lines. LOBs produced in S. exigua larvae and C-OBs produced in Sf9, Tni-High Five, or Se-UCR cells were used in this experiment. OBs were directly used for ODV purification or first heated at 80°C for 40 min before dissolving in DAS at 20°C. Purified ODVs were analyzed by 10% SDS-PAGE, and Western blot analysis was performed with P74 MAb. The full-length P74 and P74-N fragments are indicated by arrows.
