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P74 cleavage is conserved in L-OBs produced in two different hosts. L-OBs were purified from infected S. exigua and T. ni larvae. Sf9-C-OBs were included as a control. OBs were either treated with DAS to purify ODVs directly or were heated at 80°C for 40 min before dissolving in DAS. Purified ODVs were separated by 10% SDS-PAGE. Western analysis was performed with P74 MAb. Arrows indicate the positions of full-length P74 and the cleaved P74-N fragment.
