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P74-N and P74-C-Flag subunits are not associated through disulfide bonding. L-ODVs of Ac-Rep-P74-Flag virus were purified from heated or nonheated L-OBs. The nonheated L-ODV sample was treated with reducing or nonreducing Laemmli buffer, and the heated L-ODVs were treated with reduced Laemmli buffer. Proteins were separated by 10% SDS-PAGE. Western analysis was performed with P74 MAb and Flag PAb. Arrows point to full-length P74-Flag, P74-N, P74-N nonreduced, and P74-C-Flag.
