Table 1.

Activation of a subset of IFN-inducible genes following treatment of primary human fibroblasts with p14(wt) and p14Δ30^a

Functional gene group	GenBank accession no.	Gene	Description of gene product	Change in expression relative to that of LF2000 with indicated treatment
				Mock	p14(wt)	p14Δ30
IFN-inducible genes	NM_001111	ADAR	Adenosine deaminase, RNA specific	−1.02	2.09	1.37
	NM_001565	CXCL10	Chemokine (C-X-C motif) ligand 10	−3.39	12.70	1.22
	NM_005101	ISG15	ISG15 ubiquitin-like modifier	−1.29	21.85	2.31
	NM_002038	IFI6	IFN-α-inducible protein 6	−1.46	150.55	9.81
	NM_005531	IFI16	IFN-γ-inducible protein 16	−1.14	2.50	1.40
	NM_005532	IFI27	IFN-α-inducible protein 27	−1.17	114.04	6.86
	NM_006332	IFI30	IFN-γ-inducible protein 30	−1.12	2.15	−1.08
	NM_005533	IFI35	IFN-induced protein 35	−1.04	5.76	1.41
	NM_006417	IFI44	IFN-induced protein 44	−1.04	10.52	2.28
	NM_006820	IFI44L	IFN-induced protein, 44-like	−1.59	151.35	9.14
	NM_022168	IFIH1	IFN-induced protein with helicase C domain 1	−1.78	10.28	2.21
	NM_001548	IFIT1	IFN-induced protein with tetratricopeptide repeats 1	1.09	60.74	6.61
	NM_001547	IFIT2	IFN-induced protein with tetratricopeptide repeats 2	−1.03	8.62	2.18
	NM_001549	IFIT3	IFN-induced protein with tetratricopeptide repeats 3	−1.19	8.66	1.76
	NM_003641	IFITM1	IFN-induced transmembrane protein 1 (9-27)	−1.03	20.31	2.64
	NM_005373	MPL	Myeloproliferative leukemia virus oncogene	2.60	4.46	4.70
	NM_002462	MX1	Myxovirus (influenza virus) resistance 1, IFN-inducible protein p78	1.87	538.21	25.21
	NM_002534	OAS1	2′,5′-Oligoadenylate synthetase 1, 40/46 kDa	1.24	1178.69	41.36
	NM_004509	SP110	SP110 nuclear body protein	−1.01	2.80	1.46
IFNs	NM_020124	IFNK	IFN-κ	1.24	4.53	1.81
IFN receptors	NM_002187	IL12B	Interleukin-12B (natural killer cell stimulatory factor 2)	1.18	3.92	1.39
NM_005373	MPL	Myeloproliferative leukemia virus oncogene	2.60	4.46	4.70
IFN regulatory factors	NM_002460	IRF4	IFN regulatory factor 4	1.24	2.46	−1.26
NM_001572	IRF7	IFN regulatory factor 7	1.26	6.77	1.54

^aRT² Profiler IFN pathway-specific arrays were utilized to investigate RNA expression profiles following treatment with p14. Human fibroblasts were treated with 4 μg of purified p14(wt) or p14Δ30 protein in the presence of LF2000 for 24 h. Genes whose expression was upregulated more than a factor of 2 relative to that of the LF2000 control in an experimental treatment group were identified.