Table 3.
Single-virion analyses of RNAs packaged by HIV-2 Gag
| Coexpressed constructsa | CeFP+ particles |
RNA labeling efficiencyc | |||
|---|---|---|---|---|---|
| Total no. analyzed | YFP+ (%) | mCherry+ (%) | YFP+ + mCherry+ (%)b | ||
| HIV-1–noGagCeFP-MS2SL + HIV-2–GagCeFP-BglSL | |||||
| Expt 1 | 2,405 | 0.0 | 92.6 | 0.9 | 93.5 |
| Expt 2 | 835 | 0.0 | 96.3 | 0.2 | 96.5 |
| Expt 3 | 1,227 | 0.0 | 92.3 | 2.1 | 94.4 |
| Expt 4 | 1,089 | 0.0 | 93.8 | 1.0 | 94.8 |
| Expt 5 | 909 | 0.0 | 97.4 | 0.9 | 98.3 |
| Expt 6 | 614 | 0.0 | 98.4 | 0.0 | 98.4 |
| Expt 7 | 1,548 | 0.0 | 91.5 | 1.2 | 92.7 |
| HIV-1–noGagCeFP-MS2SL + HIV-2G–agCeFP-BglSL-noTatRev | |||||
| Expt 1 | 495 | 0.0 | 97.0 | 0.6 | 97.6 |
| Expt 2 | 428 | 0.0 | 93.9 | 1.4 | 95.3 |
| Expt 3 | 391 | 0.0 | 98.0 | 0.3 | 98.3 |
| Expt 4 | 1,176 | 0.0 | 90.3 | 0.7 | 91.0 |
| HIV-1–DIS6C-noGagCeFP-MS2SL + HIV-2–DIS6G-GagCeFP-BglSL-noTatRev | |||||
| Expt 1 | 687 | 0.0 | 91.7 | 0.9 | 92.6 |
| Expt 2 | 527 | 0.0 | 91.7 | 0.8 | 92.5 |
| Expt 3 | 1,146 | 0.0 | 94.9 | 1.6 | 96.5 |
| Expt 4 | 810 | 0.0 | 90.6 | 1.5 | 92.1 |
a
Although not indicated, both Bgl-mCherry and MS2-YFP were coexpressed in all experiments.
b
Average ± SD for HIV-1–noGagCeFP-MS2SL + HIV-2–GagCeFP-BglSL, 0.9 ± 0.7; average ± SD for HIV-1–noGagCeFP-MS2SL + HIV-2–GagCeFP-BglSL-noTatRev, 0.8 ± 0.5; average ± SD for HIV-1–DIS6C-noGagCeFP-MS2SL + HIV-2-DIS6G-GagCeFP-BglSL-noTatRev, 1.2 ± 0.4.
c
Calculated by adding the values from the three columns under the CeFP+ heading.