Fig. 4.
Critical role of the heparin binding proexosite in the FX SP domain in Ad5 cell binding ex vivo. (A) Liver slices from MF1 mice were incubated with 2 × 109 vp of Alexa 488-labeled Ad5 in the presence of WT or SP mutant rFX. (B) Attachment of Ad5 particles to liver slices was quantified using ImageJ counting software. Data represent the average number of particles by analyzing at least 6 separate 40× microscope fields per experimental condition. *, P < 0.05. Error bars represent standard errors of the mean.